Figure 6. Effect of TNFα on TGFβ1-driven COOH-terminal phospho-Smad2 in the Araki-Sasaki-corneal epithelial cell line

A: Bands for Smad2 (60 kDa) and phospho-Smad2 were observed in cell lysate. TGFβ1 upregulates phosphorylation of Smad2 from 0.5 h with a peak at 2.0 h post-addition. Copresence of exogenous TNFα does not affect the level of phosphorylation of Smad2 up to 5.0 h post-ligand(s) addition. B-G: Immunocytochmeistry shows that nuclear accumulation of phospho-Smad2 is observed in both TGFβ1 or TGFβ1 plus TNFα cultures at 1 and 6 h. The scale bar is equal to 30 μm.