Figure 3. Expression of Msi1 mRNA in mouse eye during development and at adulthood

Semiquantitative RT-PCR was used to determine the relative amounts of Msi1 mRNA (A) in whole mouse eye at P0, P8, P15, P21, P28, and P60A and (C) in the neuroretina (Re), retinal pigmented epithelium (RPE), and ciliary body (CB) of adult mice. Cyclophilin A was used as an internal control. The 411 and 311 bp bands correspond to Msi1 and cyclophilin PCR products, respectively. B,D: Densitometric analysis of intensities of PCR bands correspond to A and C, respectively. The relative levels were calculated as the ratio of intensities of the Msi1 band to the cyclophilin band. B: The intensity of the Msi1 band increased from P8 to P15 and seemed to be stable from P15 until adulthood. D: Msi1 mRNA levels were higher in the retina than in the RPE and CB (n = 3). Error bars indicate SEM.