Table 1 of
Sengupta, Mol Vis 2007;
13:1406-1411.
Table 1. Primers and PCR conditions for amplification of SLCA5A2 exons
The primers were selected such that the amplicons contain coding sequence (exons) and the flanking splice junctions [10]. Any variation in the sequence identified by DNA sequencing was evaluated for its potential deleterious effect on the gene product (mRNA and/or protein). In case of exons 1 and 3, nested primers were used for sequencing the amplicons for better fidelity of the data generated. The asterisk and "NA" denotes not applicable.