Figure 4 of Comes, Mol Vis 2007; 13:1363-1374.


Figure 4. Effects of glucocorticoid receptor short-interfering RNA perfusion on the expression of dexamethasone-induced genes in intact human trabecular meshwork in organ cultures

Two pairs of post-mortem human eyes were perfused with either GR siRNA (OD) or scramble control (OS) at 100 nM for two days followed by perfusion with combined 100 nM DEX/siRNA for 24 h. Fresh siRNAs media were changed from the syringes every 24 h. After perfusion, trabecular meshwork tissues were dissected and processed for RNA extraction, RT reaction, and real-time TaqMan PCR using GR, MYOC, CDT6, and 18S TaqMan probes. Normalization of GR, MYOC, and CDT6 expression in each sample was achieved using the 18S TaqMan probe hybridized to its own 10-4 diluted RT. Scramble negative control samples were normalized to a value of 0 (no silencing)and GR, MYOC, and CDT6 percent silencing values from scramble are expressed as the mean±SEM. Individual 5 (top): GR, n=6, p=0.0000003; MYOC, n=6, p=0.00000001; CDT6, n=6, p=0.00001. Individual 6 (bottom): GR, n=6, p=0.0000003; MYOC, n=6, p=0.000006; CDT6, n=6, p=0.0002. Perfusion with GR siRNA significantly reduced the expression of the GR gene as well as the downstream expression of two DEX-induced genes, MYOC and CDT6, in the perfused intact human trabecular meshwork tissue.

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Comes, Mol Vis 2007; 13:1363-1374 <http://www.molvis.org/molvis/v13/a150/>
©2007 Molecular Vision <http://www.molvis.org/molvis/>
ISSN 1090-0535