Figure 2 of He, Mol Vis 2007; 13:1348-1356.


Figure 2. The effects of JWH015 on trabecular meshwork cell focal adhesion structures

Cultures of TM cells on fibronectin-coated (5 μg/ml) coverslicps were starved in DMEM for 48 h then treated for three h with vehicle (A), 100 nM JWH015 (B), 100 nM JWH015 plus 1 μM SR144528 (C), 1 μM of SR144528 (D), 100 nM JWH015 plus 1 μM SR141716A (E), or 1 μM SR141716A (F). TM cells were then fixed with paraformaldehyde and stained with an anti-paxillin antibody and a TRITC-conjugated secondary antibody as detailed in the Methods section. JWH015 treatment resulted in a significant reduction in the number of focal adhesion structures detected with anti-paxillin antibody, compared with untreated control. This effect of JWH015 was antagonized by SR144528 but not SR141716A.

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He, Mol Vis 2007; 13:1348-1356 <http://www.molvis.org/molvis/v13/a148/>
©2007 Molecular Vision <http://www.molvis.org/molvis/>
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