Figure 1. The effects of JWH015 on the trabecular meshwork cell actin cytoskeleton

TM cells were plated on fibronectin-coated (5 μg/ml) coverslips and grown to confluence. Cells were starved in DMEM for 48 h then treated for three h with vehicle (A), 100 nM JWH015 (B), 100 nM JWH015 plus 1 μM SR144528 (C), 1 μM SR144528 (D), 100 nM JWH015 plus 1 μM SR141716A (E), or 1 μM SR141716A (F). The TM cells were then fixed with paraformaldehyde and stained with Alexafluor 488-labeled phalloidin as detailed in the Methods section. JWH015 caused a significant decrease in staining for actin stress fibers, compared with untreated control. SR144528, but not SR141716A, blocked the changes brought about by JWH015.