Figure 2. Confocal immunolocalization of nerve growth factor, neurotrohin-3, and neurotrophin-4

Merged images of confocal immunocytochemical localization of nerve growth factor (NGF; green), neurotrohin-3 (NT3; green), and neurotrophin-4 (NT4; green) and Thy-1 (red) in adult primary (A-D) and transformed retinal ganglion cell-5 (RGC5) cells (F-H). B, and F show the presence of NGF in the soma (arrows) and the neurites (arrowheads) of both the primary RGCs and RGC-5 cells, respectively. NGF and Thy-1 are co-expressed in the cells. Likewise, NT3 (C and G) and NT4 (D and H) are co-expressed with Thy-1 in the soma (arrows) and neurites (arrowheads) of both cell types. The IgG controls are shown for RGCs (A) and transformed RGC-5 cells (E).