Figure 3. Protein carbonyl content in the soluble protein fraction of lens

Protein carbonyl groups of soluble protein fraction of different groups were assayed by reactivity to 2,4-DNPH as described in the Methods section. Protein carbonyl content, a measure of oxidative damage to proteins, increased in untreated diabetic (Group II) compared with controls (Group I). Emblica (Group IV) and its tannoids (Group III) prevented the alterations in protein carbonyls. Data are mean±SD (n=4). The asterisk above the bar denotes that data are significantly different from Group I and the sharp (hash mark) denotes that data are significantly different from Group II.