Table 1 of
Qiong, Mol Vis 2007;
13:1234-1244.Table 1 of
Qiong, Mol Vis 2007;
13:1234-1244.
Table 1. Reverse-transcriptase polymerase chain reaction primer sequences for mRNAs amplified
This table describes the primer sequences used in the RT-PCR experiments. T (°C)&cycles indicate the annealing temperature and number of PCR cycles for each primer set, respectively. GAPDH is glyceraldehydes-3-phosphate dehydrogenase.
Target (GenBank Product accession Sequences (5'-3') Temperature length number) (S:Sense, A: Anti-sense) (°C) Cycles (bp) ---------- ------------------------ ----------- ------ ------- GAPDH S: AGTCCACTGGCGTCTTCAC 51 31 639 (AB060340) A: GCTTGACAAAGTGGTCGTTGA M1 S: CTGGCCTGTGACCTCTGG 59 31 437 (AY072058) A: TGAGCTGCTGCTGCTGCC M2 S: AGCAATGCCTCAGTCATG 51 35 659 (AY072059) A: TTTGATGCATGTTTGCTT M3 S: AGAATCTATAAGGAAACT 48 35 506 (AY072060) A: TTTTTGAAAACTGCCGCC M4 S: CTCTGGGCGCCTGCTATC 53 35 466 (AY072061) A: GTCTCTGTGGTGGACAG M5 S: ACAGAGAAGCGAACCAAA 53 35 455 (AY072062) A: GAGTGTGTGAGCAGCAGC |