Figure 5. Pax6^+/lacZ/AP-2α^+/- adult mouse eyes

Histological examination of the adult eye sections were carried out using hematoxylin and eosin (H & E) staining as well as periodic acid schiff (PAS) staining. No defects were observed with H & E staining in the adult wild type eyes (A) and AP-2α^+/- eyes (B). While both Pax6^+/lacZ (C) and Pax6^+/lacZ/AP-2α^+/-(D) eyes demonstrated lens separation defects, only Pax6^+/lacZ/AP-2α^+/-eyes demonstrated a persistent lens stalk (D). Normal PAS staining was observed in adult wild type (E) and AP-2α^+/- (F) eyes. PAS staining indicated matrix deposition within the plaques, the lens stalk remnants of Pax6^+/lacZ (G), and the lens stalks of Pax6^+/lacZ/AP-2α^+/-(H) eyes. PAS staining also demonstrated continuity of the lens capsule around the stalk in the Pax6^+/lacZ/AP-2α^+/- eyes (H). LE, lens epithelium; CE, corneal epithelium; P, plaque; LSR, lens stalk remnant; LS, lens stalk; LC, lens capsule. All scale bars are 100 μm.