Figure 2. Colocalization of Pax6 and AP-2α in the lens transitional zone

Pax6 (red) and AP-2α (green) were found to be colocalized (yellow) in the central lens epithelium. Pax6 expression carried on further than AP-2α into the transitional zone of the lens at developmental stages E13.5 (A), E16 (B), and post-natally (C). Pax6 was detected with a rhodamine-conjugated secondary antibody and AP-2α was detected with a FITC-conjugated secondary antibody. TZ, transitional zone. All scale bars are 100 μm.