Table 2 of Lin, Mol Vis 2007; 13:1203-1214.


Table 2. Real-time reverse transcriptase polymerase chain reaction analysis of retinal G protein-coupled receptor and retinal G protein-coupled receptor splice isoform transcripts in the retina and retinal pigment epithelium of donor Y-005

The relative expression ratios were determined by the method of Pfaffl [27]. Cp values were obtained by the LightCycler 480 software 1.2.0.0625, according to the second derivative maximum method. Abbreviations: RPE represents retinal pigment epithelium, RGR represents retinal G protein-coupled receptor, GAPDH represents glyceraldehyde-3-phosphate dehydrogenase.

                                                 Expression      Copy
                  Cp (mean±SEM)                     ratio        number
         ------------------------------------   ------------   ---------
Tissue       RGR        RGR-d         GAPDH     RGR    RGR-d   RGR-d/RGR
------   ----------   ----------   ----------   ----   -----   ---------
RPE      32.63±0.05   33.87±0.09   35.02±0.19   28.4   15.5      0.17

Retina   30.10±0.06   30.54±0.05   27.61±0.02   1      1         0.33

Lin, Mol Vis 2007; 13:1203-1214 <http://www.molvis.org/molvis/v13/a131/>
©2007 Molecular Vision <http://www.molvis.org/molvis/>
ISSN 1090-0535