Table 2 of
Lin, Mol Vis 2007;
13:1203-1214.
Table 2. Real-time reverse transcriptase polymerase chain reaction analysis of retinal G protein-coupled receptor and retinal G protein-coupled receptor splice isoform transcripts in the retina and retinal pigment epithelium of donor Y-005
The relative expression ratios were determined by the method of Pfaffl [27]. Cp values were obtained by the LightCycler 480 software 1.2.0.0625, according to the second derivative maximum method. Abbreviations: RPE represents retinal pigment epithelium, RGR represents retinal G protein-coupled receptor, GAPDH represents glyceraldehyde-3-phosphate dehydrogenase.
Expression Copy Cp (mean±SEM) ratio number ------------------------------------ ------------ --------- Tissue RGR RGR-d GAPDH RGR RGR-d RGR-d/RGR ------ ---------- ---------- ---------- ---- ----- --------- RPE 32.63±0.05 33.87±0.09 35.02±0.19 28.4 15.5 0.17 Retina 30.10±0.06 30.54±0.05 27.61±0.02 1 1 0.33 |