Table 2 of
Lu, Mol Vis 2007;
13:1154-1160.Table 2 of
Lu, Mol Vis 2007;
13:1154-1160.
Table 2. Primers for mutation screening of CRYBA1
Shown are primers for polymerase chain reaction and sequencing of exon 1-6 of CRYBA1.
Product Annealing
Exon Forward primer (5'>3') Reverse primer (5'>3') size temperature
---- ----------------------- ---------------------- ------- -----------
1 AGAGGGAGAGCAGAGTGCTG TCTTCTCCAGCCACCCTAGA 234 bp 64 °C
2 TCGTGTGTGCTCTGTCTTCC CCCCTACAAACTGGGGTTTT 205 bp 64 °C
3 CTAGCTCTCTTGCGCCATTC GAGCCTAGGGGAACATCCTT 254 bp 64 °C
4 GCTCTACTGGGATTGGCTTG GTCCCATCCCTCATATGCAC 309 bp 62 °C
5 TTTCTCACAAATCTGTTGCCTTA TGCAACCTCAGGAGTTACTTTG 340 bp 62 °C
6 AGGCTCAGGTTTTGGGGTAT ACTCCAGCCTGAGCAACAAG 471 bp 62 °C
|