Figure 4 of
Mitchell, Mol Vis 2007;
Figure 4. Western blot and reverse transcription polymerase chain reaction analysis of the expression patterns of RhoA, Rac1, and Cdc42 in different compartments of the adult mouse eye
A: Western blot analysis of RhoA, Rac1, and Cdc42 in cornea, lens, and retina. Note the differential expression patterns of three small GTPases in cornea, lens, and retina. While only Rac1 was expressed in the lens, all three small GTPases were expressed in both cornea and retina. B: Western blot analysis of Rac1 in lens epithelium and fiber cells. Expression of Rac1 is predominant in epithelium but much less so in lens fiber cells. C: RT-PCR analysis of RhoA, RhoB, Rac1 and Cdc42 in cornea, lens epithelium, lens fibers and retina. Note that the mRNA for the four small GTPases were all expressed in relatively high levels in retina and slightly lower levels in the cornea. In the lens, while Rac1 was expressed in both epithelial cells and fiber cells, only traces of RhoA and Cdc42 mRNA were detected in the epithelium. A relatively high level of RhoA mRNA was detected in the lens fiber cells. However, RhoA protein is not detectable, indicating its translational control. Similarly, some Cdc42 mRNA was also detectable in the fiber cells although Cdc42 protein is not detected. RhoB mRNA is absent in both epithelium and fiber cells of the adult lens.