Figure 4. Inhibition of mitogen-activated protein kinase pathways affects neural retina leucine-zipper-mediated transactivation of rhodopsin minimal promoter in transfected CV-1 cells

CV-1 cells were co-transfected with NRL, CRX, or combination of both to transactivate bovine rhodopsin minimal promoter (BR130-luc) in vitro. Luciferase expressed in each reaction condition was normalized with the corresponding amount of b-galactosidase and represented as relative light unit in the histogram. The DMSO treated samples are represented as, black bar and treated as control in the experiment. Whereas, samples treated with kinase inhibitor, U0126 and SB203580 represented as shaded white and shaded brown, respectively. Data from three independent experiments were used to calculate mean and standard error for each experimental condition and students' t-test was used to determine the statistical significance between different treatment groups. Values marked with * represents statistically significant at p<0.001 in the analysis.