Table 2 of
Guyon, Mol Vis 2007;
13:1094-1105.Table 2 of
Guyon, Mol Vis 2007;
13:1094-1105.
Table 2. Semiquantitative polymerase chain reaction marker characteristics and specific conditions
Markers designed from the six genes of interest were amplified together with aldolase gene marker (ALDO) as a control using the indicated conditions. The opsin gene marker (RHO) was amplified in the same manner in order to estimate the retinal integrity. U indicates upper primer; L indicates lower primer.
Primers MgCl2
Size conc. conc.
Name Primer U Primer L (bp) (μM) (mM)
---------- ---------------------- -------------------- ---- ---------- -----
RPGRIP-rg2 CTGAAGCCAGTGAAGCACAA TCCACGAGGTCTCCTGATTC 610 0,12 3,5
RANBP-rg2 TTCGAAACAGCTGTCAAGAAAC CAGGTTTGTCCACAGTTCCA 421 0,12 3,5
NPM-rg1 CAACACATTCTTGGCAATGG CAGCCAAAAATGCACAAAAA 435 0,12 5
PDE6D-rg1 GGGATGCTGAGACAGGAAAG CCCAAAACCCAAATTCTTGA 426 0,12 5
NPHP-rg1.2 AGGCCATCTCTCACGGAATA CTTTCCCTTCTGCCTCCTTC 365 0,6 3
ABCR-rg2 CCATGCTAAGGAAGCTGCTC GTGGTGTCCCCAGTAAGCAT 490 0,12 2,5
RHO-rg2 CTGTGGTCTTTGGTGGTCCT AGCAGATCAGGAAAGCGATG 421 0,04 2,5
ALDO-rg3 ATCCTGGCTGCAGATGAGTC ATAGGATGaCACCCCCAATG 151 0,12 2.5-5
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