Table 1 of
Yang, Mol Vis 2007;
13:1083-1093.Table 1 of
Yang, Mol Vis 2007;
13:1083-1093.
Table 1. Oligonucleotides used for reverse transcription polymerase chain reaction
Primers were designed using the Primer Express Program and purchased from AuGCT Biotechnology, Beijing, P. R. China.
Annealing
temperature
Target gene Sequences (5'-3') Location (°C)
----------- --------------------------- ----------- -----------
β-Actin F: CATCCTGCGTCTGGACCT NT 603-1082 59
R: TCAGGAGGAGCAATGATCTTG
MCP-1 F: ACTGGACCAGAACCAAGTGAGA NT 342-666 61
R: TGTTGAACCAGGATTCACAGAGA
MCP-3 F: GGTTGAGGAGGCCATAGCATAC NT 329-705 61
R: CACTGATTCTTGCAATGTCCCT
MIP-1α F: GAGCTGGAACTAAATGCCTGA NT 328-653 61
R: TCACCAAACACAGTGTGAGCA
MIP-1β F: CCTTCTGCGATTCAGTGCTGT NT 47-256 61
R: CATACTCATTGACCCAGGGCT
Eotaxin F: GCACGCTGAAAGCCATAGTCT NT 204-557 61
R: CATCTCCTTCCATGCTCTCTCCT
Rantes F: CTGCATCCCTCACCGTCATC NT 17-242 57
R: CACTTCTTCTCTGGGTTGGCA
IL-10 F: AGAAGCTGAAGACCCTCTGGA NT 385-693 59
R: TGGAGAGAGGTACAAACGAGGT
iNOS F: GCTCCATGACTCTCAGCACAG NT3164-3509 57
R: CTCAACCTGCTCCTCACTCAAG
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