Figure 1. Phase-contrast photomicrographs of the cultured retinal microglial cells

A: Mixed cell cultures from newborn Sprague-Dawley rats for 14 days. B: Purified cells cultured for 48 h. Cells developed the characteristic ramified shape with a long single process and a small cell soma (arrow). C: Purified cells stimulated with LPS for 24 h became bigger and rounder, showing an ameboid shape (arrowhead). Pretreatment with minocycline D or sulforaphane E for 1 h before LPS administration partly inhibited LPS-induced microglial morphological change, with some cells sustaining their ramified shape (arrow), but a few cells changing to an ameboid shape (arrowhead). F: Pretreatment with SB203580 for 1 h before LPS administration completely inhibited the LPS-induced microglial morphological change, with almost all cells sustaining their ramified shape (arrow).