Table 2 of
Gu, Mol Vis 2007;
13:1045-1057.
Table 2. List of primers and cycling conditions for RT-PCR
1Amplification conditions were 1 min/94 °C, 1.5 min/57 °C, 1.5 min/72 °C for 32 cycles. 2Amplification conditions were 1 min/94 °C, 1.5 min/56°C, 1.5 min/72 °C for 30 cycles.
Product size Gene Primer sequence (5'-3') (bp) -------------- ----------------------------- ------- Nestin1 F: GGCTTCTCTCAGCATCTTGG R: AAGGCTGGCATAGGTGTGTC 150 β-tubulin III2 F: CAGAGCAAGAACAGCAGCTACTT R: GTGAACTCCATCTCGTCCATGCCCTC 250 GFAP1 F: TTGACCTGCGACGTGGAGTC R: AGGTGGCGATCTCGATGTCC 225 β-actin2 F: CTTCCCCTCCATCGTGGG R: GTGGTACGGCCAGAGGCG 355 |