Table 2 of
Gu, Mol Vis 2007;
13:1045-1057.Table 2 of
Gu, Mol Vis 2007;
13:1045-1057.
Table 2. List of primers and cycling conditions for RT-PCR
1Amplification conditions were 1 min/94 °C, 1.5 min/57 °C, 1.5 min/72 °C for 32 cycles. 2Amplification conditions were 1 min/94 °C, 1.5 min/56°C, 1.5 min/72 °C for 30 cycles.
Product
size
Gene Primer sequence (5'-3') (bp)
-------------- ----------------------------- -------
Nestin1 F: GGCTTCTCTCAGCATCTTGG
R: AAGGCTGGCATAGGTGTGTC 150
β-tubulin III2 F: CAGAGCAAGAACAGCAGCTACTT
R: GTGAACTCCATCTCGTCCATGCCCTC 250
GFAP1 F: TTGACCTGCGACGTGGAGTC
R: AGGTGGCGATCTCGATGTCC 225
β-actin2 F: CTTCCCCTCCATCGTGGG
R: GTGGTACGGCCAGAGGCG 355
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