Figure 6. Quantification of E60 retina-derived retinal progenitor cells and 3 week old ciliary epithelium-derived retinal stem cells displaying distinct immunoreactivity after serum-induced differentiation in vitro

Dissociated retinal progenitor cell (RPC) and retinal stem cell (RSC) spheres were incubated in differentiation medium for two weeks, fixed, and immunostained with the indicated antibodies. Quantification was performed by recording the number of immunopositive cells over the number of nuclei counterstained with PI in random fields. Two hundred-1,000 cells for each immunostaining reaction for each culture were counted. Differentiated cells manifested retinal neural phenotypes in different proportions as indicated. A relatively large percentage of cells in both cultures displayed GFAP immunoreactivity. Data represent the mean±SD of three independent experiments.