Table 1 of
Gamundi, Mol Vis 2007;
13:1031-1037.Table 1 of
Gamundi, Mol Vis 2007;
13:1031-1037.
Table 1. Primers and conditions used for mutation detection
Within the table, (GC) represents: 5'- CCG CCG CGC CCG CGC CCG GCC CGC CGC CCC CGC CCG-3'. DGGE means denaturing gradient gel electrophoresis. In DGGE gradient column, 100% denaturant equals to 7M urea and 40% (v/v) formamide.
Annealing Amplicon
temperature size
Primer Sequence* (5'-3') (°C) (bp) DGGE gradient
------- ---------------------------- ----------- -------- --------------
Exon 1A
F: (GC)-GGAAGCAACCCGGACTACAC 60 379 40-70%
R: TAGCCAGGTACGGCTTCAGC
Exon 1B
F: (GC)-ATTGCATGGAAGCCCTG 60 379 40-70%
R: TCTGACCCCAGGACTGGAAG
Exon 2
F: (GC)-AAGCCCATCTCCAGCTGT 63 353 45-75%
R: CTTACCCTCTACCCCCAGCTG
Exon 3
F: AGATTGCCTCTAAATCTCCT 54 294 -
R: GGAGTGCACTATTTCTCAGT
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