Figure 5 of
Karring, Mol Vis 2007;
13:997-1004.
Figure 5. Immunoblotting of albumin/IgG-depleted human plasma using the specific anti-TGFBIp134-236 antiserum
The immunoblot shows increasing amounts of albumin/IgG-depleted human plasma in 20 mM Tris-HCl (pH 7.4) and 100 mM NaCl. Each lane contains 20 μl of diluted albumin/IgG-depleted plasma. Lanes 1-5 contain increasing amounts of albumin/IgG-depleted human plasma. The indicated volumes refer to the equaling amount of crude plasma. Lane 6 contains 0.2 μg of purified porcine TGFBIp, which was used as a control. Lane 7 is the molecular weight standard.