Figure 3 of
Karring, Mol Vis 2007;
13:997-1004.Figure 3 of
Karring, Mol Vis 2007;
13:997-1004.
Figure 3. Purification of recombinant NH2-terminal TGFBIp fragment
The His-tagged NH2-terminal TGFBIp-fragment (residues Gly134-Ile236) was expressed in E. coli and purified from the insoluble fraction using Ni2+-affinity chromatography. The step-wise elution was analyzed by SDS-PAGE and the gel was stained with coomassie brilliant blue. Lane 1 shows the eluate from elution buffer containing 40 mM imidazole. Lane 2 contains the eluate from 60 mM imidazole. Lane 3 contains the eluate from 100 mM imidazole. All lanes contain 50 μl of the elution.
