Figure 1. Nerve growth factor, trkA^NGFR, p75^NTR and αSMA expression by vernal keratoconjunctivitis-derived fibroblasts

A: Conventional RT-PCR. showing from left to right, nerve growth factor (NGF; 120 bp), trkA^NGFR (103 bp); p75^NTR (100 bp) amplicons. The lines are as follows: (1) -RT, (2) healthy-FBs, and (3) VKC-FBs. These are representative gels from three independent experiments where equal amounts of cDNA were amplified. B: Confocal microscopy on VKC-FBs showing, from left to right, that VKC-FBs express p75^NTR (Cy3, red) and trkA^NGFR (Cy2, green). p75^NTR and trkA^NGFR colocalized in some cellular compartments (merge; X600/oil immersion). C: Confocal microscopy on VKC-FBs showing, from left to right, that these cells express p75^NTR (Cy3, red) and αSMA (Cy2, green). p75^NTR and αSMA colocalized in some cellular compartments (merge; X600/oil immersion).