Figure 1.

GFP expression of normal conjunctiva in vitro and in vivo. When the cultured human subconjunctival fibroblasts (SCFs) were coinfected with adenoviral vectors encoding Cre under control of the CAG promoter and GFP under control of Cre-LoxP system, GFP was observed on day 2 and later in cytoplasm and nucleous in human SCFs. No fluorescence was detected in cultured human SCFs with CAG-Cre vector only (A). As for gene introduction in vivo, GFP was detected in conjunctival fibroblasts and epithelium of normal mice without mechanical injury at day 2 and later (until 14 days). No fluorescence was detected in normal eyes with CAG-Cre vector only (B).