Figure 1 of Park, Mol Vis 2006; 12:832-840.


Figure 1.

Expression of full length myocilin (A), myocilin 1-270 (B), and myocilin 271-504 (C) proteins in Rosetta2(DE3)pLysS. The bacteria were grown in LB medium without (lanes 1-5) or with (lanes 7-11) supplementation of 1.0% glucose (LB + 1% Glc). Protein expression was then induced in 0.2 mM IPTG. Aliquots for SDS-PAGE analyses were from uninduced control (U), and samples (I) induced for 0.5, 1, 2, or 3 h. Proteins were visualized by Coomassie blue staining. Arrows denote the induced proteins. Lane 6 shows molecular weight markers (M). The size of marker proteins from the top is 120, 100, 90, 80, 70, 60, 50, 40, 30, and 25 kDa, respectively.

(111 K)

Park, Mol Vis 2006; 12:832-840 <http://www.molvis.org/molvis/v12/a94/>
©2006 Molecular Vision <http://www.molvis.org/molvis/>
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