Table 1 of
Ito, Mol Vis 2006;
12:802-810.Table 1 of
Ito, Mol Vis 2006;
12:802-810.
Table 1.
Primer pairs used in semiquantitative reverse trasncriptase polymerase chain reaction.
Annealing
temperature
Gene Forward primer (5'-3') Reverse primer (5'-3') (°C) Cycles
-------- ---------------------- ------------------------- ----------- ------
GAPDH GAAACCCATCACCATCTTCCAG AAGGTGGAAGAATGGGAGTTGC 55 25
Asns ACAGACAGCGTGGTGATCTTCT ACTTGTAATGGGTCAGAGTGCG 55 32
c-jun CAATCTGAAGCCGCACCTCCGA TTCCTCTCCGCCTTGATCCGCT 61 36
EPCR ACACACACGCTGGAAGGC GCAGTTCATACCGAGTCCG 51 35
Fgl AGTGGGACCTACAGAGTTACG CTGGGTGTGCCTGACTT 51 35
IL1β TATGTCTTGCCCGTGGAGCTT TCCCGACCATTGCTGTTTCCTA 58 36
MCP-1 ACGCTTCTGGGCCTGTTGTT CTCTGTCATACTGGTCACTTCTACA 55 36
MIC-1 TCTCCCCGAAGCCTACCGCGT GGGTGTAGCTGGAGGGGACACA 55 37
MT-1 TCCTGCTCCACCGGC AGGCTTTTATTATTCACATGCTCGG 55 30
MT-2 ACTGCCGCCTCCATTC ATAGAAAAAAGTGTGGAGAACC 55 30
NMDMC AGATGGCCTCCTTGTTCAGCT TGTAACCAGCTTTCTTCTTGACTCC 55 33
Sestrin1 TGCAGAATTTAGGAGAACTT AAGCAGATTAACATGAACCT 55 33
TSP-1 ATGCCTGTCCAATCCCTGCTTT CATCCTTGTCATAGTCTTCCTG 55 30
|