Table 1 of
Robinson, Mol Vis 2006;
12:704-711.
Table 1.
The Lampi et al. [2] previously reported percentages were normalized as described (The average of four donors, 0, 3, 4, and 7 days of age, were measured by scanning of 2-D gels). All crystallins in these samples and the 10 measured herein by FTMS were soluble. Only soluble proteins were analyzed because insoluble proteins were negligible due to the young ages of the donors. The 2 Day column shows a combination of four 2-day-old donors. Median and mean are mole percentages. Standard deviation is percent standard deviation of the mean value, so, mean=29.3 and percent standard deviation=11% represents mean=29.3±3.2. The 23 day thru 300 day column shows the results of five 23, 25, 30, 150, and 300-day-old donors. The 2 day thru 300 day column shows the data from the combination of all nine donors. The 90 day column shows data indicating that the 90-day-old donor may represent an unusual biological variant. The asterisk indicates that γS-crystallin co-migrated with βA1-crystallin for 15.4+% total. The double asterisks indicate that βA2-crystallin was not detected. The sharps (hash mark) indicate that βB3-crystallin co-migrated with βA3-crystallin for 6.2+% total.
2 day 23 day-300 day 2 day-300 day 0, 3, 4, ----------------------- ----------------------- ----------------------- Crystallin Mass 7 day [2] Median Mean % St Dv Median Mean % St Dv Median Mean % St Dv 90 Day ---------- ----- --------- ------ ---- ------- ------ ---- ------- ------ ---- ------- ------ Alpha A 19939 25.5 29.5 29.3 11 19.5 20.8 14 24.3 24.6 22 22.7 Alpha B 20188 7.4 6.6 6.3 11 6.8 6.7 17 6.6 6.6 14 14.1 Gamma D 20594 2.9 5.8 5.5 29 7.7 7.8 9.2 7.1 6.7 24 8.6 Gamma C 20734 16.4 12.1 12.1 15 11.3 11.8 14 11.3 11.9 14 17.5 Gamma S 20904 Gamma S/ 8.6 8.6 8.6 8.9 8.8 5.4 8.9 8.7 6.7 13.1 Beta A1* Beta A2 21993 nd** 1.6 1.6 0.8 0.9 34 0.9 1.0 40 1.2 Beta A4 22271 5.0 6.7 6.5 17 5.2 5.4 59 6.3 5.9 41 8.6 Beta A3 22631 1.7 1.7 1.7 13 1.7 1.7 37 1.7 1.7 30 3.4 (23-215) Beta A1 23087 Gamma S/ 2.9 2.3 54 2.5 2.3 31 2.6 2.3 37 Beta A1* Beta B2 23276 14.6 12.7 13.4 21 19.5 19.6 19 17.3 16.8 27 3.8 Beta B3 24209 Beta A3/ 1.5 1.5 1.6 1.5 25 1.5 1.5 22 Beta B3# Beta A3 25176 Beta A3/ 3.0 3.4 51 4.2 3.8 30 3.2 3.6 38 Beta B3# Beta B1 26518 2.0 3.0 3.1 12 2.5 2.4 24 2.8 2.7 22 4.2 (16-251) Beta B1 27917 7.5 8.2 8.1 14 6.7 6.5 41 7.8 7.3 30 8.6 |