Table 1 of Robinson, Mol Vis 2006; 12:704-711.


Table 1.

The Lampi et al. [2] previously reported percentages were normalized as described (The average of four donors, 0, 3, 4, and 7 days of age, were measured by scanning of 2-D gels). All crystallins in these samples and the 10 measured herein by FTMS were soluble. Only soluble proteins were analyzed because insoluble proteins were negligible due to the young ages of the donors. The 2 Day column shows a combination of four 2-day-old donors. Median and mean are mole percentages. Standard deviation is percent standard deviation of the mean value, so, mean=29.3 and percent standard deviation=11% represents mean=29.3±3.2. The 23 day thru 300 day column shows the results of five 23, 25, 30, 150, and 300-day-old donors. The 2 day thru 300 day column shows the data from the combination of all nine donors. The 90 day column shows data indicating that the 90-day-old donor may represent an unusual biological variant. The asterisk indicates that γS-crystallin co-migrated with βA1-crystallin for 15.4+% total. The double asterisks indicate that βA2-crystallin was not detected. The sharps (hash mark) indicate that βB3-crystallin co-migrated with βA3-crystallin for 6.2+% total.

                                          2 day                23 day-300 day             2 day-300 day
                     0, 3, 4,    -----------------------   -----------------------   -----------------------
Crystallin   Mass    7 day [2]   Median   Mean   % St Dv   Median   Mean   % St Dv   Median   Mean   % St Dv   90 Day
----------   -----   ---------   ------   ----   -------   ------   ----   -------   ------   ----   -------   ------
 Alpha A     19939   25.5         29.5    29.3    11        19.5    20.8    14        24.3    24.6    22        22.7
 Alpha B     20188    7.4          6.6     6.3    11         6.8     6.7    17         6.6     6.6    14        14.1
 Gamma D     20594    2.9          5.8     5.5    29         7.7     7.8     9.2       7.1     6.7    24         8.6
 Gamma C     20734   16.4         12.1    12.1    15        11.3    11.8    14        11.3    11.9    14        17.5
 Gamma S     20904   Gamma S/      8.6     8.6     8.6       8.9     8.8     5.4       8.9     8.7     6.7      13.1
                     Beta A1*
 Beta A2     21993   nd**          1.6     1.6               0.8     0.9    34         0.9     1.0    40         1.2
 Beta A4     22271    5.0          6.7     6.5    17         5.2     5.4    59         6.3     5.9    41         8.6
 Beta A3     22631    1.7          1.7     1.7    13         1.7     1.7    37         1.7     1.7    30         3.4
 (23-215)
 Beta A1     23087   Gamma S/      2.9     2.3    54         2.5     2.3    31         2.6     2.3    37
                     Beta A1*
 Beta B2     23276   14.6         12.7    13.4    21        19.5    19.6    19        17.3    16.8    27         3.8
 Beta B3     24209   Beta A3/      1.5     1.5               1.6     1.5    25         1.5     1.5    22
                     Beta B3#
 Beta A3     25176   Beta A3/      3.0     3.4    51         4.2     3.8    30         3.2     3.6    38
                     Beta B3#
 Beta B1     26518    2.0          3.0     3.1    12         2.5     2.4    24         2.8     2.7    22         4.2
 (16-251)
 Beta B1     27917    7.5          8.2     8.1    14         6.7     6.5    41         7.8     7.3    30         8.6

Robinson, Mol Vis 2006; 12:704-711 <http://www.molvis.org/molvis/v12/a79/>
©2006 Molecular Vision <http://www.molvis.org/molvis/>
ISSN 1090-0535