Table 3 of
Allison, Mol Vis 2006;
12:655-672.Table 3 of
Allison, Mol Vis 2006;
12:655-672.
Table 3. Assessment of ICAT/MS techniques reproducibility by analyzing different peptides that were identified from individual proteins
The table shows proteins that were identified multiple times from different peptides. The quantification of multiple peptides that may have originated from the same protein allow one to assess the reliability of the data. We report a T:C ratio, where T represents the abundance of the peptide in thyroid hormone treated retina, and C represents the abundance in the control retina. A T:C ratio represents the fold increase (if>1) or fold decrease (if<1) of the reported peptide in the retina of trout treated with thyroid hormone. The column labeled "Conf" is the confidence.
Number
peptides
Name detected Accession ID Peptide Conf Score T:C Avg T:C St Dev
----------------------------- -------- ------------ ------------------------- ---- ----- ----- ------- ------
β tubulin 5 gi|13324679 LTTPTYGDLNHLVSATMSGVTTCLR 99 50 0.968 0.833 0.152
MREIVHLQAGQCGNQIGAK 99 42 0.947
TAVCDIPPR 99 48 0.867
NMMAACDPR 90 33 0.791
EIVHLQAGQCGNQIGAK 99 65 0.592
Transferrin 5 gi|5837759 SHNEPYYDYAGAFQCLK 75 24 1.134 1.011 0.087
CLVEGAGDVAFIK 90 34 0.994
ETNNCDFTTYFSK 50 16 0.985
CHDLAANVAQFSCVR 99 51 0.929
IECQDAPTVDECIKK 99 36
Glycogen phosphorylase, brain 4 gi|1730559 SSKFGCR 50 16 1.471 1.083 0.267
form TCAYTNHTVLPEALER 99 40 1.050
INMAHLCVIGSHAVNGVAR 99 36 0.919
QLLNCLHIITLYNR 90 30 0.894
α-tubulin 4 gi|340019 CDPGHGKYMACCLLYR 90 31 1.128 0.941 0.128
AYHEQLSVADITNACFEPANQMVK 90 34 0.865
SIQFVDWCPTGFK 90 32 0.919
YMACCLLFR 75 22 0.853
Similar to guanine nucleotide 3 gi|27881927 LFVSGACDASAK 99 42 0.598 0.583 0.018
binding protein LLLAGYDDFNCNVWDTLK 90 32 0.588
ADQELMVYSHDNIICGITSVAFSK 99 38 0.562
Pyruvate kinase 3 gi|21038972 AGKPITCATQMLESMIK 99 38 0.987 0.912 0.068
AEGSDVANAVLDGADCIMLSGETAK 90 31 0.896
NTGIICTIGPASR 99 46 0.853
Sodium/potassium-transporting 3 gi|18858309 SSVTWVALARVAALCNR 50 16 1.205 1.107 0.138
ATPase ACVIHGTDLK 99 46 1.009
CIELSSGSVK 50 17
Phosphopyruvate hydratase β 3 gi|105934 SGETEDTFIADLVVGLCTGQIK 99 50 0.845 0.754 0.096
VNQIGSVTESIQACK 99 52 0.764
ACNCLLLK 75 22 0.654
Creatine kinase 3 gi|6016429 LGYILTCPSNLGTGLR 99 42 1.032 1.201 0.365
VCAEARDQHK 75 22 0.951
HNNCMASHLTPAVYAK 99 54 1.619
V-type ATPase B subunit 2 gi|4929105 TACEFTGDILR 99 44 1.002 0.942 0.119
GPTVLAEDYLDIMGQPINPQCR 50 16 0.883
EAA44879 2 gi|30177396 YMSCCLLYR 90 28 0.950 0.934 0.030
SIQFVDWCPTGFK 90 32 0.919
Cellular nucleic acid-binding 2 gi|29423610 DCEQTEDACYNCHR 50 18 0.986 0.935 0.102
protein CGEIGHVAVQCSK 99 50 0.884
Similar to phosphorylase 2 gi|28422226 VKEFCENLPADGR 50 16 1.019 0.925 0.186
phosphatase LNIISNLDCVNEVIGIR 99 50 0.832
RIKEN cDNA 3110065L21 2 gi|25072051 IHEGCEEPATHNALAK 99 42 0.955 0.866 0.177
EDKLECSEELGDLVK 75 26 0.778
Arrestin 2 gi|2308986 VYVMLSCTFR 75 25 0.690 0.653 0.075
CAVEFEVK 75 24 0.615
SI:bZ18K17.1 2 gi|22204199 GILLFGPPGCGK 99 42 1.068 0.967 0.203
LGANSGLHIIIFDELDAICK 90 34 0.866
Glutamine synthetase 2 gi|21666321 LVLCEVLK 90 29 0.941 0.903 0.077
RPSANCDPYAVTEALIR 99 56 0.864
Creatine kinase, testis 2 gi|125314 FCTGLTK 75 21 1.076 0.977 0.199
isozyme GFCLPPHCSR 99 48 0.877
Guanine nucleotide-binding 2 gi|121009 ELPGHTGYLSCCR 75 20 1.094 0.912 0.363
protein G(I) LLLAGYDDFNCNIWDAMK 50 16 0.731
Chain A, Structure of the 2 gi|11513661 HHCPNTPIILVGTK 90 30 1.621 1.228 0.786
RacP67PHOX complex CVVVGDGAVGK 99 39 0.835
Rhodopsin 2 gi|10720158 YIPEGMQCSCGIDYYTR 99 36 0.983 0.788 0.392
WIVVCKPISNFR 99 48 0.592
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