Figure 1. Construction and purification of DNA tethered magnetic nanoparticles

Gel electrophoresis was used to examine the successful construction of the DNA tethered magnetic nanoparticles and to verify that the purification removed the unbound DNA from the nanoparticles. Lanes A-C represent only DNA fragments. Lanes D-F contain the MNP/DNA mixture. After washing with a magnetic column, a portion of the purified MNP were also run on this gel (Lanes G-I). On the right is a magnified image of the box around Lanes F-I. This gel shows three critical stages of nanoparticle construction, DNA alone, DNA tethered nanoparticle in excess DNA, and finally DNA tethered nanoparticles free of unbound DNA.