Table 2 of
Szabo, Mol Vis 2006;
12:597-605.Table 2 of
Szabo, Mol Vis 2006;
12:597-605.
Table 2.
Primer sequences, codes, annealing temperature, and anticipated size of the amplified product for the different fragments of lumican and keratocan gene studied (LUM: NM_002345; KERA: NM_007035). During PCR, the forward primer containing the GC-clamp (F-GC) was the 40-mer 5'-GCG GCC GCC GCG TCC CGC CGC CCC CGC CCC GCC GCG GCC G-3' and the reverse primer containing the GC-clamp (R-GC) was the 40-mer 5'-CGG CCG CGG CGG GGC GGG GGC GGC GGG ACG CGG CGG CCG C-3'.
Annealing Fragment
temperature length
Fragments Primer 5'-3' (°C) (bp)
------------------ ---------------------------------- ----------- --------
LUM exon 1 F CTTTCTCCCCACGTTCACCT 58 353
LUM exon 1 R ATTGGCAAAATTGCTCTGGA
LUM exon 2.1 F ATGTTGCAAATTGAATGTCTT 56 421
LUM exon 2.1 R CCCTTTTATCTTGGAGTTTTCTA
LUM exon 2.2 F AAGGCCTTTGAGAATGTAACTG 56 409
LUM exon 2.2 R TGTTGCTGATCTTATTGTTGTCTA
LUM exon 2.3 F CTGCCTTCTGGTCTCCCTGTCTCT 65-60-58 428
LUM exon 2.3 R CCACTTCGGCCTCCCAAAATG
LUM exon 3 F TGGTTGCAAATTCAAACATATAC 60 499
LUM exon 3 R ATTCGTGTATATGTGTGTGTTC
KERA exon 1.1 F TATCACATTTACATTTTTTC 50 354
KERA exon 1.1 R-GC GC clamp-AAATTCTTACTTTAATCCT
KERA exon 1.2 F TTTGCTGGGCTGTGTAGGCAC 54 494
KERA exon 1.2 R-GC GC clamp-CGGGTTTTGGTTTAGTTATTG
KERA exon 2.1 F ACATATTTTCACCTCTTCCC 58 668
KERA exon 2.1 R-GC GC clamp-AGGCATTGTCCACTAATTTG
KERA exon 2.2 F-GC GC clamp-TGGAAGATAATGAGCTAGAGGAG 58 617
KERA exon 2.2 R TAAAGGAACATTAGCGGGGG
KERA exon 3 F-GC GC clamp-TTTCATATATTAGGCCACTAAGCCC 58 447
KERA exon 3 R CTGGCAAAAGCATCTTTGAATAG
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