Figure 2 of
Szabo, Mol Vis 2006;
A: Determination of optimal melting temperature by perpendicular electrophoresis on polyacrylamide gel. B: Parallel running of KERA 2.1 exon at optimal melting temperature. Lanes 1 and 2 show PCR amplicons of control patients, while lanes 3-9 present amplicons of patients with persistent corneal haze. Separated DNA strands of heteroduplexes caused by an intronic polymorphism were detected (arrows) in lane 2. Homoduplexes were visualized in lanes 1,3-9.