Figure 2 of Szabo, Mol Vis 2006; 12:597-605.

Figure 2.

A: Determination of optimal melting temperature by perpendicular electrophoresis on polyacrylamide gel. B: Parallel running of KERA 2.1 exon at optimal melting temperature. Lanes 1 and 2 show PCR amplicons of control patients, while lanes 3-9 present amplicons of patients with persistent corneal haze. Separated DNA strands of heteroduplexes caused by an intronic polymorphism were detected (arrows) in lane 2. Homoduplexes were visualized in lanes 1,3-9.

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Szabo, Mol Vis 2006; 12:597-605 <>
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