Table 1 of
Hunter, Mol Vis 2006;
12:588-596.Table 1 of
Hunter, Mol Vis 2006;
12:588-596.
Table 1.
PCR primer sequences used to place cataract orthologs on canine chromosomes using an RH5000 panel. The column labeled "Tm" indicates primer melting temperature. PCR product size and corresponding NCBI reference sequences are shown.
GenBank
accession Tm Product
Gene number Primer sequences (°C) size (bp)
------ --------- --------------------------- ---- ---------
Structural protein genes
BFSP2 NM_003571 F: CCTCTTCATAGTTCCTTGAC 50.7 419
R: CCCTACAGTTCTATGCTTCCAG 55.8
CX46 NM_021954 F: CCAGAGAATGCTAATTTGTCCC 54.9 365
R: CAGCACATGCACTTACACAGG 57.6
CX50 NM_005267 F: GGGATAACCAGAGGGTAGCAC 57.8 386
R: GGATCTCGAAGTTCTCCTACC 54.9
LIM2 NM_030657 F: GGCAACAAGTGCTACCTGC 56.9 353
R: CCCAGTTTCCAACCTAGTTC 53.0
MIP NM_012064 F: GGGCACGAGTACGAGCACTG 61.0 308
R: GCCTGCACGCTTCGCAAGC 63.5
SPARC NM_003118 F: CTGATCATGTGGCCTTGAGC 56.2 343
R: GGCCTAATCTGATCTGCTAAG 52.5
Crystallin genes
CRYBB2 NM_000496 F: GGAGAGCAGTTTGTGTTCGAG 55.9 356
R: GTGCAATGTGGCAACCCTTAG 56.9
CRYGA NM_014617 F: CCTACGAAGGAATCCCTTTTG 53.2 349
R: GAGTGGAGCTCATTGAGCCG 58.1
CRYGB NM_005210 F: GCTCCTGGCATCCAGTGGG 61.0 404
R: CTGCTCCTGCTGCTCTACTC 57.3
CRYGC NM_020989 F: CTCTAACCCAGACTCTGAACC 54.3 373
R: GGACCCAGCAGCCCTCCAG 63.3
CRYGD NM_006891 F: GACAGCGGCTGCTGGATGC 62.1 381
R: CCAGGACAGGACCTATTGCTG 57.4
CRYGS NM_017541 F: CAGCTCATCCCAAGACTGAATG 55.7 390
R: GACACCATCCAGCACCTTACTG 57.7
Transcription factors
CHX10 NM_182894 F: CGTAAGTTAAGTACCAGAGGG 52.1 390
R: CTAAACCTGTGATTTCTGTGCC 54.0
EYA1 NM_172060 F: CTACTGCCCAAACTCATCAC 53.2 386
R: CCCACAGAAAGAATGTAGATG 50.7
FOXE3 NM_012186 F: GCAAGTCACTGCAGGGACTG 58.6 342
R: GTTCTTTAGTGGTGCTGGAGG 55.5
HSF4 NM_001538 F: GATTCCTGAGCTCTACCATAG 51.7 463
R: GCCAGGGTCTGGTTGAAGC 58.9
MAF NM_005360 F: GTTAACAGCACGGAGGTTACAC 55.9 340
R: CCCTTCCACTGCATCTGAGTC 57.6
PAX6 NM_001604 F: GCCACATCTTCAGTACAAAG 49.6 300
R: TAGTTCAGGCATTGACTGATG 50.3
PITX3 NM_005029 F: CCTCCCTGAACAGGGTGATAG 57.5 304
R: CCGCACCATTGCACACCACG 63.0
SIX5 NM_175875 F: GACCCGCAGCTTCTCAAGC 59.0 371
R: CCTTCCTACTGCAAAGTGAGC 55.8
SOX1 NM_005986 F: CTCAAGAATGATATCCACTGCTTC 53.5 299
R: GTACATTTCAGAGTCAATGTGGC 54.1
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