Figure 3 of
Sreelakshmi, Mol Vis 2006;
Intrinsic tryptophan fluorescence spectra for wild-type αB- and αBinvert-crystallin mutant. Protein samples in phosphate buffer were excited at 295 nm, and emission spectra were recorded from 300 nm to 400 nm. The fluorescence intensity of αBinvert-crystallin mutant was 50% lower than that of the wild-type.