Figure 2.

Molar mass distribution and elution profile of wild-type αB- and αBinvert-crystallin mutant. Wild-type αB- and αBinvert-crystallin mutant (0.2 mg each) in phosphate buffer were injected. Chromatography and molecular weight determination were performed as described in methods and elsewhere [9]. The αBinvert-crystallin mutant formed a smaller oligomer than wild-type αB-crystallin.