Table 1 of
Yang, Mol Vis 2006;
12:511-517.
Table 1. B16LS9 VEGF and PEDF mRNA levels after supplemental angiostatin
In vitro the level of VEGF and PEDF mRNA (mean±SEM) was determined by real time RT-PCR. When B16LS9 cells were grown in vitro in the presence of the supplemental angiostatin the level VEGF and PEDF mRNA and ratio of VEGF to PEDF mRNA level (mean±standard deviation) was less than when they were grown in media alone. VEGF and PEDF mRNA values are normalized to β-actin according to Pfaffl's mathematical model for relative quantification in real time PCR [23].
Groups VEGF/PEDF VEGF/β-actin PEDF/β-actin ----------- ------------- ------------- ------------- Angiostatin 0.8751±0.1106 0.1324±0.0292 0.1513±0.0459 PBS 2.6994±0.1453 0.2275±0.1089 0.0850±0.0428 ----------- ------------- ------------- ------------- p value 0.00006 0.2176 0.1420 |