Table 1 of Yang, Mol Vis 2006; 12:511-517.


Table 1. B16LS9 VEGF and PEDF mRNA levels after supplemental angiostatin

In vitro the level of VEGF and PEDF mRNA (mean±SEM) was determined by real time RT-PCR. When B16LS9 cells were grown in vitro in the presence of the supplemental angiostatin the level VEGF and PEDF mRNA and ratio of VEGF to PEDF mRNA level (mean±standard deviation) was less than when they were grown in media alone. VEGF and PEDF mRNA values are normalized to β-actin according to Pfaffl's mathematical model for relative quantification in real time PCR [23].

Groups        VEGF/PEDF       VEGF/β-actin    PEDF/β-actin
-----------   -------------   -------------   -------------
Angiostatin   0.8751±0.1106   0.1324±0.0292   0.1513±0.0459
PBS           2.6994±0.1453   0.2275±0.1089   0.0850±0.0428
-----------   -------------   -------------   -------------
p value       0.00006         0.2176          0.1420

Yang, Mol Vis 2006; 12:511-517 <http://www.molvis.org/molvis/v12/a60/>
©2006 Molecular Vision <http://www.molvis.org/molvis/>
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