Figure 2 of
Barren, Mol Vis 2006;
12:492-498.
Figure 2. Time courses of GTPγS binding to Gtα* and Gtα*R238E
The binding of GTPγS to Gtα* (rectangle, triangle) and Gtα*R238E (down triangle, diamond; 1 μM each) in the presence of 2 μM Gtβγ (rectangle, down triangle) or 2 μM Gtβγ and uROS membranes (50 nM rhodopsin; triangle, diamond) began with the addition of 20 μM [35S]GTPγS. Gα-bound GTPγS was counted by withdrawing aliquots at the indicated times and passing them through Whatman cellulose nitrate filters (0.45 μm). The calculated kapp values (min-1) are 0.006 (rectangle), 0.078±0.002 (triangle), 0.007 (down triangle), and 0.088±0.002 (diamond).