Figure 2 of Layton, Mol Vis 2006; 12:43-54.


Figure 2. Immunohistochemical characteristics of cultures

Each pair of panels shows the cellular architecture of the culture: The top panel presents a hematoxylin stain and the corresponding immunolabeling is beneath it. A: Cultured Müller cells grown in hormonally defined medium (HDM) and 5 mM glucose for 24 h with no added insulin. Cells demonstrate expression of vimentin, a specific Müller cell marker, along with GFAP and FGF-2. B: Cultured retinal pigment epithelium (RPE) cells grown in HDM and 5 mM glucose for 24 h with no added insulin. Cells are immunoreactive to cytokeratin (K 8.13). C: Representative Müller cell GFAP labeling after 24 h of treatment. It can be seen that cultures in 15 mM glucose display increased GFAP production relative to cultures treated in the 5 mM glucose environment. This increase appears to be evident in all cells, however some cells appear to be more intensely labeled. D: Representative RPE cell labeling after 24 h of treatment. It can be seen that cultures in 15 mM glucose display quantitatively increased fibroblast growth factor-2 production when treated with 9 nM insulin. Subtly decreased FGF-2 labeling was seen after 24 h in cultures in 15 mM glucose without insulin. The scale bar represents 100 μm.

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Layton, Mol Vis 2006; 12:43-54 <http://www.molvis.org/molvis/v12/a5/>
©2006 Molecular Vision <http://www.molvis.org/molvis/>
ISSN 1090-0535