Table 1 of
Abu-Safieh, Mol Vis 2006;
12:384-388.Table 1 of
Abu-Safieh, Mol Vis 2006;
12:384-388.
Table 1. Markers and primers used for deletion analysis
Markers, listed in descending order from centromere, and primers used for deletion analysis. Sequence information is based on NCBI Homo sapiens chromosome 19 genomic sequence NT_011109. The nucleotide positions of single nucleotide polymorphysm (SNPs) within PRPF31 gene are based on the start codon in the PRPF31 gene (NM_015629).
Marker/SNPs Location of marker/SNP Sense primer (5'-3') Antisense primer (5'-3') Tm
----------- ---------------------- -------------------- ------------------------ --
JST060265 Proximal to OSCAR TGGGGTAGAAAGAGTATG TCTATTTTCTCCAAAGACGG 56
JST060264
JST114222 5' UTR of OSCAR GAAAGGGGTGACTCACAGAG TTCCACAGTGCCATAGGGCT 60
(GATA)n about 1.5 kb 5' of TTTGCACCTATTATGTGCCA TCACAGCAAGGGCCTGGCAC 58
NDUFA3 start ATG
420+81T>C
420+82G>C PRPF31 IVS5 GAGTCTCCTTCCATCTCACC CAGAGCAGACCACTGAGCC 59
528-31C>T PRPF31 IVS6 GGAGATCCAGGAGGCTGG GGTCACAGTGTCAGCAGACC 61
564G>A
735C>T PRPF31 exon8 CTCTCTGCTTTCTTCTGACC TGAGTGCTACCGTCAGCT 60
855+40G>A PRPF31 IVS8
946-70T>C PRPF31 IVS9 TAAGGCACGTGGATACTCG CATCTTGCGGTACCTGGG 61
1148-9T>C PRPF31 IVS11 CTGGTCGCTGAACTGCAG CCTTGCCAAGGTCTCAGG 64
1468C>T PRPF31 exon 14 CTCACCTAACCCATCATCC GCTCTGATTCTTCTAGTTGCC 55
(TGTT)n about 1.4 kb 3' GGATGATCCTGGCTCATG GAGAATCACTTCAACCTGG 57
of PRPF31 STOP
(CA)n 0.5 kb 5' of CNOT3 CCCCCAATCTTTCTTCTCCT AGCTGATGACTGGGATGGAG 59
start ATG
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