Figure 3. Reduction of vascular endothelial growth factor (VEGF) receptor levels following transfection with ribozymes

Transfection with the VEGFR-1 ribozyme reduced VEGFR-1 surface expression while also reducing VEGFR-2 levels. Transfection with VEGFR-2 ribozyme reduced VEGFR-2 levels and also VEGFR-1 levels. These results suggest a level of coregulation of VEGF receptor expression. The graph shows non-transfected HRECs (NT), HRECs transfected with the VEGFR-1 ribozyme (R1 Rz), and HRECs transfected with the VEGFR-2 ribozyme (R2 Rz), and HRECs tranfected with both the VEGFR-1 and VEGFR-2 ribozymes (R1+R2 Rz).