Figure 2 of
Spoerri, Mol Vis 2006;
12:32-42.
Figure 2. VEGFR-1 and VEGFR-2 mRNA expression is inhibited by the ribozymes
The VEGFR-1 (A) and VEGFR-2 (B) ribozymes specifically reduce levels of their respective target mRNAs. The graph shows the results of real-time PCR on cDNAs produced from total RNA isolated from nontransfected (NT) human retinal endothelial cells (HRECs), from HRECs transfected with plasmids expressing the VEGFR-1 (VEGFR-1 Rz) or VEGFR-2 (VEGFR-2 Rz) ribozymes. For each sample the level of the VEGFR-1 or VEGFR-2 mRNA was determined relative to β-actin mRNA.