Figure 1 of Hawse, Mol Vis 2006; 12:342-349.

Figure 1. Metallothionein IIa over-expression protects human lens epithelial cells against cytotoxic effects of cadmium exposure

A: Semi-quantitative RT-PCR confirmation of MTIIa over-expression in retroviral infected HLECs (MTIIa) relative to uninfected control cells (control). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) shows equal loading of RNA between the two cell lines. The results shown are representative of three experiments. Note the 3-4 fold increase in MTIIa gene expression in the MTIIa cells compared to controls. B: Representative graph depicting increased resistance to cadmium treatment of the MTIIa over-expressing cell line (dashed line) relative to control cells (solid line). Cadmium treatments were conducted for 24 h. The absorbance readings (reflecting cell viability, with larger values indicating increased numbers of live cells) and cadmium concentrations are indicated. Each error bar represents the standard deviation of eight separate cell-viability assays. Differences at 40 and 80 μM cadmium are significant at p<0.001.

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Hawse, Mol Vis 2006; 12:342-349 <>
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