Table 1 of
Ramirez-Miranda, Mol Vis 2006;
12:318-323.Table 1 of
Ramirez-Miranda, Mol Vis 2006;
12:318-323.
Table 1. Oligonucleotides used for PAX6 amplification
Sequence of oligonucleotides and annealing temperatures used for exon-by-exon PAX6 amplification, modified from [11]. In the table, "F" indicates the forward strand and "R" indicates the reverse strand.
Annealing
temperature
Exon Sequence (°C)
---- -------------------------- -----------
3 F: TCAGAGAGCCATGGAGGTAT 55
3 R: CTGTTTGTGGGGTTTTGAGCC 55
4 F: TTGGGAGTTCAGGCCTACCT 55
4 R: GAAGTCCCAGAAAGACCAGA 55
5 F: CCTCTTCACTCTGCTCTCTT 55
5 R: ATGAAGAGAGGGCGTTGAGA 55
5a F: TGAAAGTATCATCATATTTGTAG 60
5a R: GGGAAGTGGACACAAAACCA 60
6 F: GTGGTTTTCTGCCACTTCC 55
6 R: AGGAGAGAGCATTGGGCTTA 55
7 F: GAGCTGAGATGGGTGACTGTG 51
7 R: AGCATGGAAGCCCTGAGAGGA 51
8 F: GGGAATGTTTTGGTGAGGCT 55
8 R: CAAAGGGCCCTGGCTAAATT 55
9 F: GTAGTTCTGGCACAATATGG 55
9 R: GTACTCTGTACAAGCACCTC 55
10 F: GTAGACACAGTGCTAACCTG 55
10 R: CCCGGAGCAAACAGGTTTAA 55
11 F: TTAAACCTGTTTGCTCCGGG 55
11 R: TTATGCAGGCCACCACCAGC 55
12 F: CTGAAGCGGCTCTAACAGCCA 60
12 R: TAATGATCAGACTTGTTGGCA 60
13 F: CATGTCTGTTTCTCAAAGGGA 60
13 R: GAACAATTAACTTTTGCTGGCC 60
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