Figure 1. rAAV vector construction

A: Schematic diagram of the pTR.mOP500.GFP plasmid DNA used to make the rAAV2/5.mOP500.GFP virus. ITR represents AAV2 internal terminal repeats; mOP500 represents murine rod opsin regulatory sequence from -385 to +86; SD/SA represents SV40 late viral protein gene 16S/19S splice donor and acceptor signal; GFP represents coding sequence for the synthetic green fluorescence gene; Neor represents coding sequence for the neomycin resistance gene; pA1 and pA2 represents polyadenylation signals; Epo represents a tandem repeat of the polyoma virus enhancer region; Ptk represents thymidine kinase promoter of the herpesvirus, Tn5. B: Schematic diagram of mOP500 depicting the location of known cis-elements and their corresponding trans-acting factors. Cis-elements include Eopsin-1, PCE-1/Ret-1, BAT-1, NRE, and Ret-4. Transcription factors include Mash-1, CRX, ORX, RX, NRL, NR2E3, NR1D1. Negative trans-elements include Fiz-1, a zinc-finger protein that attenuates transactivation of rhodopsin promoter by interaction with NRL, and KLRF-1, a repressor.