Figure 6. Effects of noladin ether on trabecular meshwork cell actin cytoskeleton

Trabecular meshwork cells were plated on fibronectin (10 μg/ml)-coated cover glasses and grown to confluence. Cells were starved in DMEM for 24 h, and then treated for 3 h with vehicle (A), 100 nM of noladin ether (B), 100 nM of noladin ether plus 1 μM SR141716A (C), or 1 μM of SR141716A (D). The trabecular meshwork cells were then fixed and stained with Alexafluor 488-labeled phalloidin as detailed in the Methods.