Figure 5. Involvement of CB1 cannabinoid receptors in inhibition of trabecular meshwork cell adhesion to fibronectin-coated surface caused by noladin ether treatment

In the presence of 100 nM of Noladin ether (NE) alone, 100 nM NE plus one μM of SR141716A (SR1), one μM of SR1 alone, 100 nM NE plus one μM of SR144528 (SR2), or one μM of SR2 alone, TM cells were allowed to attach for three h to wells pre-coated with 10 μg/ml fibronectin. Adhered TM cells were quantified by crystal violet staining as detailed in the Methods and results are expressed as absorbance at 590 nm. Data from four different experiments are presented. Error bars represent the SEM. Asterisks denote data is significantly different from the control (vehicle; p<0.05; ANOVA with Neuman-Keuls post hoc test).