Figure 1. Effects of different concentrations of noladin ether on fibronectin-induced migration of trabecular meshwork cells

Soluble fibronectin (20 μg/ml) was added to the lower chamber to induce TM cell migration. Different concentrations of noladin ether were added to both the upper and the lower chamber. TM cells (1x10⁵) were loaded to the upper chamber and cells migrating to the lower chamber were quantified by fluorenscence readings three h later as detailed in the Methods. Results were expressed as percent of vehicle response, which was cell migration when only vehicle (no fibronectin) was added. Data from four different experiments are presented. Error bars represent the SEM. Asterisks denote data is significantly different from the control (fibronectin alone; p<0.05; ANOVA with Neuman-Keuls post hoc test).