Figure 3 of Flynn, Mol Vis 2006; 12:271-282.


Figure 3. Confocal images of intracellular ROS generation from human lens epithelial cells in galactose-maintained and galactose-free cultures

A: Confluent monolayers of HLE-B3 cells were maintained in MEM or galactose (Gal) with or without Sorbinil (ARI), 17α-estradiol, or 17β-estradiol for 3 days. HLE-B3 cells were preloaded with DCFH-DA to capture the ROS generated. The confocal images represent a randomly chosen field after 3 days of cell culture under the various experimental treatments. One set of control cells was exposed to UV light as a positive control to demonstrate effective dye loading. The scale bar represents 20 μm. B: Statistical analysis of the average fluorescence intensity from the cells shown in Panel A (n=8, p>0.05). The plus sign (+) represents a significant increase in UV light-induced fluorescence intensity relative to control cells. Error bars represent the standard error of the mean.

(78 K)

 

(15 K)

Flynn, Mol Vis 2006; 12:271-282 <http://www.molvis.org/molvis/v12/a29/>
©2006 Molecular Vision <http://www.molvis.org/molvis/>
ISSN 1090-0535