Figure 3. Myosin heavy chain mRNA isoforms and β-actin from each of 6 extraocular muscles

In the adult rats, each of the six extraocular muscles (EOMs) expressed approximately equal percentage of 2X MHC (MYH2X), 2A MHC (MYH2A), and 2B MHC (MYH2B). For competitive PCR, the initial concentration of the competitor was 1 fmol (lane 1) and 3 fold serial dilutions to 333 amoles (lane 2), 111 amoles (lane 3), 37 amoles (lane 4), 12.3 amoles (lane 5), 4.12 amoles (lane 6), 1.37 amoles (lane 7), 0.46 amoles (lane 8), 0.15 amoles (lane 9), 0.05 amoles (lane 10), 0.017 (lane 11), and 0.006 (lane 12). The upper bands of the PCR products are the targets and the lower bands are competitors. Arrowheads indicate the approximate concentration at which target and competitor are the same. In the first lane, the "L" is a 100 bp ladder with 600 bp indicated. The plus sign indicates a RT-PCR band of MHC mRNA transcript without competitor. Gels are shown for 2A MHC, 2B MHC, 2X MHC, EOM MHC (MYHC-EO), embryonic MHC (MYHC-Emb), neonatal MHC (MYHC-PN), β-cardiac MHC (MYHCB), and β-actin.