Figure 9. Western blots of ICAM-1 protein from retina and choroid

A: Total protein homogenates. Note the greater molecular weight of ICAM-1 in the choroid as compared with the retina. B: Contribution of N-linked carbohydrates to ICAM-1 molecular weight in the choroid and retina. Deglycosylation of retinal and choroidal protein results in the detection of a major band in both tissues at approximately 59 kDa. Each protein was examined with and without treatment of extract with PNGase F (N-gly).